Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis.
Salivary microRNAs (miRNAs) have been recently revealed as the next generation of non-invasive biomarkers for the diagnostics of diverse diseases.However, their short and highly homologous sequences make their quantification by RT-qPCR technique highly heterogeneous and study dependent, thus limiting their implementation for clinical applications.I